Recent peer-reviewed publications (Past 5 years)
J. S. Luterbacher, Parlange, J.-Y., Walker, L. P. 2012. A pore-hindered diffusion and reaction model can help explain the importance of pore size distribution in enzymatic hydrolysis of biomass. Biotechnology and Bioengineering, In Press
Moran-Mirabal, J. M., Bolewski, J. C. and Walker, L. P. 2012. Thermobifida fusca cellulases exhibit limited surface diffusion on bacterial micro-crystalline Cellulose. Biotechnology and Bioengineering, In Press
J. S. Luterbacher, Walker, L. P. and Moran-Mirabal, J. M.. 2012. Observing and modeling BMCC degradation by commercial cellulase cocktails with fluorescently labeled Trichoderma reseii Cel7A through confocal microscopy. Biotechnology and Bioengineering, In Press
Heavner, B. D., Smallbone, K., Barker, B., Mendes, P. and Walker, L. P. 2012. Yeast 5 - an Expanded Reconstruction of the Saccharomyces Cerevisiae Metabolic Network. BMC Systems Biology, 6: 55.
Corgie, S. C., Kahawong,P., Bowser, D., Edward, J. B., Walker, L. P. and Giannelis, E. P. 2012. Self-assembled complexes of horseradish peroxidase (HRP) with magnetic nanoparticles (MNPs) showing enhanced peroxidase activity. Advanced Functional Materials, 22, 1940 - 1951.
Luterbacher, J. S., Chew, Q., Li, Y., Tester, J. W., and Walker, L. P. 2012. Producing concentrated solutions of monosaccharides using biphasic CO2-H2O mixtures. Energy and Environmental Science, 5: 6990 - 7000.
Heavner, B. D., Henry, S. A. and Walker, L. P. 2012. Expanding sphingolipid biochemistry in the consensus genome-scale model of Yeast metabolism. Industrial Biotechnology, 8: 72 - 78.
Luterbacher, J. S., Tester, J. W., and Walker, L. P. 2012. Two-temperature stage biphasic CO2-H2O pretreatment of lignocellulosic biomass at high solid loadings. Biotechnology and Bioengineering, 109: 1499 - 1507.
Munro, S. A., Choe, L., Zinder, S. H., Lee, K. H., Walker, L. P. 2011. Proteomic and Physiological Experiments to Test Thermotoga neapolitana Constraint-Based Model Hypotheses of Carbon Source Utilization. Biotechnology Progress, 28: 312-318.
Kostylev, M., Moran-Mirabal, J. M., Walker, L. P., Wilson, D. B. 2011. Determination of the molecular states of the processive endocellulase Thermobifida fusca Cel9A during crystalline cellulose depolymerization. Biotechnology and Bioengineering, 109: 1499-1507.
Corgie, S. C. , Smith, H. M. and Walker, L. P. 2011. Enzymatic Transformations of Cellulose Assessed by Quantitative High-Throughput Fourier Transform Infrared Spectroscopy (QHT-FTIR). Biotechnology and Bioengineering, 108 (7), 1509-1520.
Zhu, P., Moran-Mirabal J. M., Luterbacher, J. S., Walker, L. P., and Craighead, H. G. 2011. Observing Thermobifida fusca Cellulase Binding to Pretreated Wood Particles Using Time-Lapse Confocal Laser Scanning Microscopy. Cellulose, 18 (3), 749-758.
Moran-Mirabal J. M., Bolewski, J. C. and Walker, L. P. 2011. Reversibility and binding kinetics of Thermobifida fusca Thermobifida fusca cellulases studied through fluorescence recovery after photobleaching microscopy. Biophysical Chemistry, 155 (1), 20-28.
Fontenelle, L. T., Corgie, S. C. and Walker, L. P. 2011. Integrating mixed microbial population dynamics into modeling energy transport during the initial stages of the aerobic composting of a switchgrass mixture. Bioresource Technology, 102: 5162 - 5168.
King, B. C., Waxman, K. D., Nenni, Ni V., Walker, L. P., Bergstrom, G. C., Gibson. D. M. 2011. Arsenal of plant cell wall degrading enzymes reflects host preference among phytopathogenic and saprophytic fungi. Biotechnology for Biofuels, 4:4.
Munro, S. A., Zinder, S. H. and Walker, L. P. 2011. Comparative constraint-based model development for thermophilic hydrogen production. Industrial Biotechnology, 7, 63-82.
Saathoff, A. J., Spanswick, R. M., Ahner, B. A., Walker, L. P. 2011. Detection of phytochelatin in the xylem sap of Brassica napus. Environmental Engineering Science, 28, 1-9.
Luterbacher, J. S., Tester, J. W. and Walker, L. P. 2010. High-solids biphasic CO2-H2O pretreatment of lignocellulosic biomass. Biotechnology and Bioengineering, 107: 451-460.
Saathoff, A. J., Spanswick, R. M., Ahner, B. A., Walker, L. P. 2009. A system for high temporal resolution of Cadmium uptake in plants. Biological Engineering, 2: 31-46.
Munro, S. A., Zinder, S. H. and Walker, L. P. 2009. Fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production. Biotechnology Progress, 25: 1035-1042.
Moran-Mirabal, J. M., Corgie, S. C. , Bolewski, J. C., Smith, H. M., Cipriany, B. R., Craighead, H. G.,Walker, L. P. 2009. Labeling and purification of cellulose-binding proteins for high resolution fluorescence applications. Analytical Chemistry, 81: 7981-7987.
Walker, L. P. 2009. Managing complexity and diversity in evolving bioenergy systems. ME Today, 11.
King, B., Donnelly, M., Bergstrom, G., Walker, L. P., Gibson. D. 2008. An optimized microplate assay system for quantitative evaluation of plant cell wall degrading enzyme activity of fungal culture extracts. Biotechnology Bioengineering, 102: 1033-1043.
Moran-Mirabal, J. M., Santhanam, N., Corgie, S. C., Craighead, H. G., and Walker, L. P. 2008. Immobilization of Cellulose Fibrils on Solid Substrates for Cellulase Binding Studies Through Quantitative Fluorescence Microscopy. Biotechnology Bioengineering, 101(6): 1129-1141.
Reccius,C. H., Stavis, S. M., Mannion, J. T., Walker, L. P. and Craighead, H. G. 2008. Conformation, length and speed measurements of electrodynamically stretched DNA in nanochannels. Biophysical Journal, 95(1): 273-286.
Santhanam, N. and Walker, L. P. 2008. A High-Throughput Assay to Measure Cellulase Binding and Synergism in Ternary Mixtures. ASABE Biological Engineering Journal, 1(1):1-19.
Pryor, S. W., Siebert, K. J., Gibson, D. M., Gossett, J. M. and Walker, L. P. 2007. Modeling Production of Antifungal Compound and their Role in Biocontrol Product Inhibitory Activity. J. Agric. Food Chem., 55: 9530-9536.
Krause, E.L., Villa-Garcia, M.J., Henry, S.A. and Walker, L.P. 2007. Determining the Effects of Inositol Supplementation and the opi1 Mutation on Ethanol Tolerance of Saccharomyces cerevisiae. Industrial Biotechnology, 3(3):260-268.
Stavis, S. M., Corgie, S. C., Cipriany, B. R., Craighead H. G. and Walker L. P. 2007. Single Molecule Analysis of Bacterial PCR Products in Submicrometer Fluidic Channels. Biomicrofluidics, 1:1-13.
Pryor, S. W., Gibson, D. M., Hay, A. G., Gossett, J. M. and Walker, L. P. 2007. Optimization of Spore and Antifungal Lipopeptide Production during the Solid State Fermentation of Bacillus subtilis. Appl. Biochem. Biotechnol. 143:63-79.
Yu, L-X, Gray, B. N., Rutzke, C. J., Walker, L. P., Wilson, D. and Hanson. M. 2007. Expression of Thermostable Microbial Cellulases in the Chloroplasts of Nicotine-Free Tobacco. J. Biotechnology, 131:362-369.
Pryor, S. W., Gibson, D. M., Bergstrom, G. C., and Walker, L. P. 2007. Minimization of Between-well Sample Variance of Antifungal Activity Measurements Using a High-Throughput Screening Microplate Bioassay. BioTechniques: 151-156.
Selected presentations (Past 5 years)
Renewable Fuels Roundtable, Subcommittee of Governor David A. Paterson's Renewable Energy Task Force, June 13, 2008, Syracuse, NY.
From Field to Fuel: Growing Biofuels in NY State. Environmental Business Association of NY State, Biofuels Industries Conference, May 29, 2008. Saratoga Springs, NY.
Industrial Ecology, A presentation to Undersecretary Gale Buchanan, USDA, May 14, 2008, Washington, DC.
Catalyzing the Development of a Domestic Biofuels Sector, Cornell Engineering Alumni Association Annual Conference, Sustainable Energy Systems: Investing in our Future, March 28, 2008, Ithaca, NY.
University/Industry Partnerships, Upstate Sustainability Symposium: Focus on Energy, Jan. 31, 2008, Pittsford, NY.
Research and Market Evolution, opportunities for NY in the alternative energy arena, NYS Agricultural Society Annual Meeting and Agricultural Forum, Jan. 10, 2008, Liverpool, NY.
Walker, L.P. 2007. Nanobiotechnology as an Enabler for Biofuels Research and Development. The New York Academy of Sciences eBriefings, The 8th Annual Nanobiotechnology Symposium, Cornell University, Ithaca, NY. www.nyas.org/nbtc8
A View from Cornell on the Evolving Biofuels Industry, Presented at the Bi-annual Meeting of the American Council on Renewable Energy, Higher Education Committee, Nov. 28, 2007, Washington, DC.
The Evolving Paradigm of Agriculture and Forestry as a Supplier of Energy, Presented at the State of New York Dept. of Agriculture and Markets, Advisory Council on Agriculture, Nov. 5, 2007, Morrisville, NY.
Transitioning to a Sustainable Global Energy Future, Presented at the 2007 Corning Energy Summit, Global Energy Management, Sept. 18, 2007, Corning, NY.
Nanotechnology as an Enabler for Biofuels Research and Development, Presented at the 8th Annual Nanobiotechnology Symposium, Oct. 1, 2007, Cornell University, Ithaca, NY.
Evolving the Paradigm of Agriculture as a Supplier of Energy and Chemicals, Presented at the University Industry Consortium Spring Meeting, April 19, 2007, Rochester, NY.
Cornell Cellulosic Biofuels Research Team, Presented to Representatives from Shell Oil, April 11, 2007, Cornell Campus.
The Evolving Paradigm of Agriculture as a Supplier of Energy and Industrial Raw Materials, Congresswoman Gillibrand's Town Hall Meeting, Feb., 19, 2007, Rhinebeck, NY.
The Evolving Paradigm of Agriculture as a Supplier of Energy and Industrial Raw Materials, Presented at the New York State Association of Counties Legislative Conference, Jan. 29, 2007, Albany, NY.
Book Chapters
Moran-Mirabal, J. M., Craighead, H.G., and Walker, L.P. 2010. Single Molecule Fluorescence Techniques for Biomedical Applications, in J.M.K. Irudayaraj (Ed.). Biomedical Nanosensors. Stanford Press. 300pp.
Patents
Cornell University/Stephane C. Corgie et al. International Application No. PCT/US12/28392 Entitled: MESOPOROUS CATALYSTS OF MAGNETIC NANOPARTICLES AND FREE-RADICAL-PRODUCING ENZYMES, AND METHODS OF USE
Corgie, S.C., Kahawong, P., Giannelis, E., Walker, L.P. 2011. "Magnetic Bionanocatalysts (BNCS) for Enhanced Generation of Free-Radical Aromatic Mediators." Provisional Application filed - Serial No. 61/451,360.
Luterbacher, J. S., Tester, J. W., and Walker, L. P. "High-Solids Biphasic CO2-H2O Pretreatment of Lignocellulosic Biomass." Provisional Application filed - Serial No. 61/319,086.
Villa-Garcia, M.J., Krause, E.L., Walker, L.P., Henry, S.A. 2007. "Methods to improve alcohol tolerance of microorganisms." Provisional patent application Docket No. 19603/5790 (CRF D-4159-01).
Wilson, D. B., Walker, L. P. and Zhang, S. 1997. "Thermostable cellulase from a thermomonospora gene." United State Patent No. 5,677,151.